Dispersive Liquid–Liquid Microextraction (DLLME) and LC-MS/MS Analysis for Multi-Mycotoxin in Rice Bran: Method Development, Optimization and Validation

Rice bran, a by-product of the rice milling process, has emerged as functional food and being used in formulation healthy drinks. However, bran is often contaminated with numerous mycotoxins. In this study, method to simultaneous detection aflatoxins (AFB1, AFB2, AFG1, AFG2), ochratoxin A (OTA), deoxynivalenol (DON), fumonisins (FB1 FB2), sterigmatocystin (STG), T-2 toxin, HT-2 diacetoxyscirpenol (DAS) zearalenone (ZEA) was developed, optimized validated using dispersive liquid–liquid microextraction (DLLME) liquid chromatography-tandem mass spectrometry (LC-MS/MS). DLLME, solvent mixture methanol/water (80:20, v/v) chloroform extraction addition 5% salt improved recoveries (63–120%). The developed further response surface methodology (RSM) combined Box–Behnken Design (BBD). Under experimental conditions, good linearity obtained correlation coefficient (r2) ? 0.990 limit (LOD) between 0.5 50 ng g?1. ranged from 70.2% 99.4% an RSD below 1.28%. proposed successfully applied analyze multi-mycotoxin 24 samples.